產品英文名稱：Protein A ELISA kit
產品中文名稱：蛋白質 A ELISA 試劑盒

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Protein A ELISA kit 的應用
本試劑盒是用于來自于自然（金黃色葡萄球菌）蛋白質配體和大腸桿菌重組表達構建結構的定量測定。該試劑盒只用于研究和制造，不用于人類或動物的診斷應用。 Cygnus制造兩個其他蛋白質的檢測試劑盒，貨號F050H和F400。F050H優于F050產品抗體,當人類或其他顯示干擾蛋白質檢測的時候。我們 的新的蛋白試劑盒F400將超過前兩包的一些改進，旨在更好地發現新的蛋白質，如被GE衛生保健出售的不自然的MabSelect SuReTM配體。

Protein A ELISA kit 的英文描述
Protein A immobilized on various chromatography media is commonly used to purify antibodies. Even when covalently attached, Protein A can leach off of the chromatography support and co-elute with the antibody. For some applications such as the pharmaceutical use of the antibody, contamination with。Protein A must be minimized to avoid any adverse effects. There are several manufacturers of Protein A and Protein A chromatography supports. In addition to natural Protein A purified from S. aureus, there are also various recombinant constructs of Protein A typically expressed in E. coli. Some of these recombinant Protein A’s are essentially identical to natural Protein A. However, there are other unnatural recombinant constructs with very significant structural differences when compared to natural Protein A. GE Healthcare sells one such unnatural construct marketed as MabSelect SuRe TM. Because of the unique structure of MabSelect SuRe TM it is only about 20% reactive in our F050 and F050H kits. For this reason weoffer our newest Protein A kit, Cat # F400. That kit incorporates a new antibody as well as other changes allowing it to detect all forms of Protein A equally. Some product antibodies when complexed with the leached Protein A can interfere in the detection of Protein A when using the F050 kit. Like our F050H kit, Cat # F400 also utilizes a sample treatment step involving acid dissociation and heat denaturation to overcome any interference from product immunoglobulin. The Cygnus Technologies’ Protein A ELISA kits are designed to detect Protein A contamination to less than one part per million. As such, these kits can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

蛋白質 A ELISA 試劑盒的使用步驟規則
The Protein A assay is a two-site immunoenzymetric assay. Samples containing Protein A are reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second biotinylated Monoclonal anti-Protein A antibody is reacted forming a sandwich complex of solid phase antibody-Protein A-biotinylated antibody. After a wash step to remove any unbound reactants, the strips are then reacted with Streptavidin labeled with Alkaline Phosphatase enzyme which will bind to any of the biotinylated antibody bound to the strip. After another wash step to remove unbound Streptavidin: alkaline phosphatase, the plates are then reacted with pnitrophenyl phosphate (PNPP) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of Protein A present. Accurate quantitation is achieved by comparing the signal of unknowns to Protein A standards assayed at the same time.

Protein A ELISA kit 的保存和穩定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.
* Reconstituted wash solution is stable until the expiration date of the kit.

Protein A ELISA 試劑盒的使用注意事項
*只用于研究或制造。
*在使用這種濃度的試劑時，其他試劑不被認為是有害的。
*本試劑盒只能由合格的技術人員使用。

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